Plant bacterial diseases are a major factor in economic crop losses worldwide.

The transportation and use of clean, pathogen-free seed and propagative material is the most vital step in controlling bacterial diseases, but this requires accurate, rapid, sensitive, and reliable pathogen detection.

The second edition of Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material is an essential, easy-to-use lab manual describing and illustrating the latest methods for detecting a wide range of bacteria responsible for many of the world’s most economically significant bacterial plant diseases in crops.

Yet this book does more than describe and illustrate the use of real-time PCR, rapid PCR-based assays, and other methods for specific, sensitive detection of bacteria in seed and propagative material. Its 48 chapters offer background information on the given bacteria and the diseases they cause, describe symptoms, detail their epidemiology, identify strategies for disease management, and provide protocols for detection in thorough detail.

The second edition of this comprehensive reference, published more than 25 years after the first edition, is organized into four parts:

• General Seed Pathology

• Detection of Plant-Pathogenic Bacteria in Cereals, Grain, Legumes, Grasses, and Forage

• Detection of Plant-Pathogenic Bacteria in Vegetables

• Detection of Plant-Pathogenic Bacteria in Vegetatively Propagated Crops

In addition to serving as a vital resource for seed companies, quarantine operations, and diagnostic labs, this book is an excellent laboratory guide for classroom instruction. It can also help facilitate rapid technology transfer in developing countries, where opportunities to receive training in this important field are rare.

Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material, Second Edition

PART I: General Seed Pathology

CHAPTER 1

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Bacterial Diseases of Plants: Epidemiology and Management Strategies

M’Barek Fatmi and Hasan Bolkan

CHAPTER 2

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Taxonomy of Plant-Pathogenic Bacteria

Norman W. Schaad, Erin Schuenzel, and Jeffrey B. Jones

CHAPTER 3

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Review of Seed Sample Size Determination and Methods for Extracting Phytopathogenic Bacteria from Seeds

Ron R. Walcott and Ron D. Gitaitis

PART II: Detection of Plant-Pathogenic Bacteria in Cereals, Grain, Legumes, Grasses, and Forage

CHAPTER 4

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Detection of Pseudomonas syringae pv. syringae in Wheat Seeds

Siham Asaad, David C. Sands, and S. Krishna Mohan

CHAPTER 5

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Detection of Xanthomonas translucens in Wheat Seeds

Etienne Duveiller and Claude Bragard

CHAPTER 6

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Detection of Pantoea stewartii subsp. stewartii in Maize Seeds

Charles C. Block, Lisa M. Shepherd, and Jerald K. Pataky

CHAPTER 7

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Detection of Clavibacter michiganensis subsp. nebraskensis in Maize Seeds

Lisa M. Shepherd, Charles C. Block, and Anne K. Vidaver

CHAPTER 8

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Detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seeds

Casiana M. Vera Cruz, Bart Cottyn, Marian Hanna Nguyen, Jillian Lang, Valérie Verdier, Twng Wah Mew, and Jan E. Leach

CHAPTER 9

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Detection of Acidovorax oryzae in Rice Seeds

Tingchang Zhao, Wei Guan, Laixin Luo, Jianqiang Li, Jianjun Feng, and Norman W. Schaad

CHAPTER 10

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Detection of Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans in Common Bean Seeds

Robert L. Gilbertson, Michael O’Leary, Irina V. Agarkova, and Anne K. Vidaver

CHAPTER 11

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Detection of Pseudomonas syringae pv. phaseolicola in Bean Seeds

S. Krishna Mohan, Yvette Berthier-Schaad, and Norman W. Schaad

CHAPTER 12

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Detection of Curtobacterium flaccumfaciens pv. flaccumfaciens in Bean Seeds and in Seeds of Other Leguminosae Crops

Stefania Tegli, Matteo Cerboneschi, and Anne K. Vidaver

CHAPTER 13

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Detection of Pseudomonas savastanoi pv. glycinea in Soybean Seeds

Lisa M. Shepherd and Charles C. Block

CHAPTER 14

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Detection of Rhodococcus fascians in Chickpea Seeds

Irina V. Agarkova and Anne K. Vidaver

CHAPTER 15

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Detection of Rathayibacter spp. in Seeds of Cereals and Grasses

Elena Postnikova, Irina V. Agarkova, William L. Schneider, Aaron J. Sechler, and Ian T. Riley

CHAPTER 16

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Detection of Clavibacter michiganensis subsp. insidiosus in Alfalfa Seeds

Deborah A. Samac, Kathy Ophel-Keller, and David Caffier

PART III: Detection of Plant-Pathogenic Bacteria in Vegetables

CHAPTER 17

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Detection of Clavibacter michiganensis subsp. michiganensis in Tomato Seeds

M’Barek Fatmi, Hasan Bolkan, and Norman W. Schaad

CHAPTER 18

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Detection of Pseudomonas syringae pv. tomato in Tomato Seeds

Parm Randhawa, Prakash Pradhanang, and Norman W. Schaad

CHAPTER 19

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Detection of Xanthomonas spp. in Tomato and Pepper Seeds

Sally A. Miller, Jeffrey B. Jones, and Chester Kurowski

CHAPTER 20

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Detection of Xanthomonas hortorum pv. carotae in Carrot Seeds

Lindsey J. du Toit, Robert L. Gilbertson, Margreet Asma, and Norman W. Schaad

CHAPTER 21

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Detection of Pantoea spp. in Onion Seeds

Teresa A. Coutinho and Ron R. Walcott

CHAPTER 22

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Detection of Burkholderia cepacia in Onion Planting Materials and Onion Seeds

Ali M. Zaid, Jo Ann E. Asselin, and Steven V. Beer

CHAPTER 23

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Detection of Xanthomonas campestris pv. campestris in Crucifer Seeds

Harrie Koenraadt, Deborah L. Hailstones, Alexander Ignatov, and Norman W. Schaad

CHAPTER 24

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Detection of Pseudomonas Pathogens from Crucifer Seeds

Carolee T. Bull and Steven T. Koike

CHAPTER 25

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Detection of Xanthomonas campestris pv. vitians in Lettuce Seeds

Steven T. Koike and Robert L. Gilbertson

CHAPTER 26

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Detection of Acidovorax citrulli in Cucurbit Seeds

Ron R. Walcott, Jianjun Feng, and Ron D. Gitaitis

CHAPTER 27

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Detection of Pseudomonas syringae pv. lachrymans in Cucumber Seeds

Carmen N. Mortensen and M’Barek Fatmi

CHAPTER 28

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Detection of Pantoea ananatis in Melon Seeds

Kazutaka Kido and Yuichi Takikawa

PART IV: Detection of Plant-Pathogenic Bacteria in Vegetatively Propagated Crops

CHAPTER 29

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Detection of Clavibacter michiganensis subsp. sepedonicus in Potato Tubers

Solke H. De Boer, John G. Elphinstone, and Norman W. Schaad

CHAPTER 30

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Detection of Ralstonia solanacearum in Potato and Geranium

X. Li, Solke H. De Boer, Anne M. Alvarez, and Norman W. Schaad

CHAPTER 31

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Detection of Common Scab–Causing Streptomyces in Potato Tubers and Soil

Leslie A. Wanner and M’Barek Fatmi

CHAPTER 32

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Detection of ‘Candidatus Liberibacter solanacearum’ in Potato

Wenbin Li, Jorge A. Abad, Aimin Wen, Neil C. Gudmestad, Jacob A. Price, and Charlie M. Rush

CHAPTER 33

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Detection of Pectobacterium spp. and Dickeya spp. in Potato Tubers

Solke H. De Boer, Amy O. Charkowski, and Jan M. van der Wolf

CHAPTER 34

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Detection of Xanthomonas fragariae in Strawberry Planting Stock

Parm Randhawa and Edwin Civerolo

CHAPTER 35

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Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus Planting Materials

Yongping Duan and Wenbin Li

CHAPTER 36

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Detection of Xanthomonas citri subsp. citri in Citrus

U. Lertsuchatavanich, J. Cubero, N. Thaveechai, and P. Llop

CHAPTER 37

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Detection of Xylella fastidiosa in Grape, Almond, and Citrus

M’Barek Fatmi, Jianchi C. Chen, and Norman W. Schaad

CHAPTER 38

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Detection of Xylella fastidiosa subsp. multiplex in Peach, Plum, and Shade Trees

C. J. Chang, Frank Wong, and Paul Vincelli

CHAPTER 39

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Detection of Xylella fastidiosa subsp. sandyi in Oleander

Donald A. Cooksey and Hamid R. Azad

CHAPTER 40

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Detection of Erwinia amylovora in Asymptomatic Budwood of Pear, Apple, and Quince

M’Barek Fatmi and Kerik D. Cox

CHAPTER 41

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Detection of Pseudomonas syringae pv. actinidiae in Kiwifruit Plants

Angelo Mazzaglia and Giorgio M. Balestra

CHAPTER 42

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Detection of Pseudomonas savastanoi pv. savastanoi in Asymptomatic Olive Plants

Stefania Tegli, Matteo Cerboneschi, and M’Barek Fatmi

CHAPTER 43

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Detection of Agrobacterium spp. in Grapevines

Thomas J. Burr, Kameka L. Johnson, Supaporn Kaewnum, and Erno Szegedi

CHAPTER 44

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Detection of Leifsonia xyli subsp. xyli in Sugarcane

Michael P. Grisham and Jeffrey W. Hoy

CHAPTER 45

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Detection of Xanthomonas albilineans in Sugarcane

Freddy Fernando Garces, Jeffrey W. Hoy, and Michael P. Grisham

CHAPTER 46

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Detection of Rhodococcus fascians in Ornamental Plants

Melodie L. Putnam

CHAPTER 47

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Detection of Agrobacterium tumefaciens in Ornamental Plants

Melodie L. Putnam

CHAPTER 48

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Detection of Burkholderia gladioli in Orchids

Lisa Keith and Piyarat Thammakijjawat

"...is an essential, easy-to-use lab manual describing and illustrating the latest methods for detecting a wide range of bacteria responsible for many of the world's most economically significant bacterial plant diseases in crops."
—Library Bookwatch

Publish Date: 2017
Format: 8.5” × 11” softcover
ISBN: Print: 978-0-89054-539-3
Online: 978-0-89054-541-6
Pages: 360
Images: 205 images
Publication Weight: 4 lbs