Plant pathogenic bacteria identification has seen many advances, most notably polymerase chain reaction (PCR). This book features a list of available PCR primers and an appendix on Molecular Techniques. Nomenclatural changes included in this manual make identification of most plant pathogenic bacteria much easier. Many species have been given genus status and many pathovars have been returned to species rank. Each chapter in this book provides a description of the nomenclature and taxonomy being followed and a flow chart to aid in identification of plant pathogen genera is included.

To assist readers in correctly identifying bacteria, the Laboratory Guide for Identification of Plant Pathogenic Bacteria, Third Edition includes simplified methods, color photographs of biochemical results, full descriptive methods and a list of semiselective agar media useful for isolating bacteria. This guidebook will be a useful resource for educators, students, researchers, extension personnel, diagnosticians or anyone involved in identifying plant bacterial diseases.

Use the updated information in this comprehensive, standardized lab guide to easily and accurately identify plant pathogenic bacteria.

Laboratory Guide for Identification of Plant Pathogenic Bacteria, Third Edition

I. Initial Identification of Common Genera

N.W. Schaad

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Genera
Diagnostic Media and Tests
Literature Cited
Chemicals List

II. Gram-Negative Bacteria

Agrobacterium

L.W. Moore, H. Bouzar, and T. Burr

Introduction
Isolation Techniques Using Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Erwinia and Pantoea

S.H. De Boer, D.L. Coplin, and A.L. Jones

Introduction
Literature Cited

Erwinia amylovora Group

A.L. Jones and K. Geider

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Erwinia Soft Rot Group

S.H. De Boer and A. Kelman

Introduction
Isolation Techniques Using Semiselective Media
Differentiation of Commonly Isolated Species and Subspecies
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Pantoea

D.L. Coplin and C.I. Kado

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Pseudomonas

A. Braun-Kiewnick and D.C. Sands

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species and Pathovars
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Acidovorax and Xylophilus

J.B. Jones, R.D. Gitaitis, and N.W. Schaad

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species and Subspecies
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Burkholderia

W. Chun and J.B. Jones

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Ralstonia

T.P. Denny and A.C. Hayward

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species and Subspecies
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Xanthomonas

N.W. Schaad, J.B. Jones, and G.H. Lacy

Introduction
Identification of Xanthomonadin Pigment
Isolation Techniques Using Differential and Semiselective
Differentiation of Commonly Isolated Species and Pathovars
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Xylella fastidiosa

D. Hopkins

Introduction
Isolation Techniques Using Differential Media
Differentiation of Commonly Isolated Pathotypes
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Rhizomonas suberifaciens

L.E. Datnoff and R.T. Nagata

Introduction
Isolation Techniques Using Semiselective Medium
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Culture Preservation
Literature Cited
Chemicals List

III. Gram-Positive Bacteria

Coryneform Plant Pathogens

M.J. Davis and A.K. Vidaver

Introduction
Isolation Techniques Using Semiselective Media
Differentiation of Commonly Isolated Species and Subspecies
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Streptomyces

R. Loria, C.A. Clark, R.A. Bukhalid, and B.A. Fry

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular Techniques
Culture Preservation
Literature Cited
Chemicals List

Bacillus

W. Chun and A.K. Vidaver

Introduction
Isolation Techniques Using Differential Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Commercial Automated Techniques
Culture Preservation
Literature Cited
Chemicals List

Clostridium

B.M. Lund and A. Kelman

Introduction
Isolation Techniques Using Differential and Semiselective Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Inculation Tests for Maceration of Tissue
Literature Cited
Chemicals List

IV. Fastidious Phloem-Limited Bacteria

M.J. Davis

Introduction
Isolation Techniques Using Selective Agar Media
Differentiation of Commonly Isolated Species
Diagnostic Media and Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Culture Preservation
Literature Cited

V. Cell-Wall Free Bacteria

I.M. Lee, R.E. Davis, and J. Fletcher

Introduction
Isolation Techniques
Differentiation of Species or Groups
Diagnostic Tests
Pathogenicity Tests
Molecular, Serological, and Commercial Automated Techniques
Literature Cited
Chemicals List

VI. Appendix

Molecular Techniques

F.J. Louws and D.A. Cuppels

Introduction
DNA Extraction
PCR-Based Methods
REP-PCR Genomic Fingerprint Analysis to Characterize and Identify Phytobacteria
Literature Cited
Diagnostic Companies

DNA Isolation Procedure

F.J. Louws

Introduction

Serological Techniques

A. Alvarez

Immunodiagnostic Methods
Literature Cited
Diagnostic Companies

Automated Techniques

E.R. Dickstein, J.B. Jones, and D.E. Stead

Physiological and Biochemical Methods
Molecular Methods
Commercial Laboratories
Literature Cited
Diagnostic Companies

“This book puts together very useful practical information for laboratories working with plant pathogenic bacteria. This book was a great help for me, especially when I started my own lab, since it became a very used resource for my students to learn how to work with some bacteria new to us.”
—Leonardo De La Fuente, APS PRESS Senior Editor and Auburn University Professor

“At last! A comprehensive source on modern plant pathogenic bacteria is now available. This is a must purchase for any applied plant pathologists and all clinical situations.”
—New Zealand Journal of Crop and Horticultural Sciences

“All persons working with plant pathogenic bacteria will find this third edition of the well known laboratory guide as a great assistance in the accurate identification of plant pathogenic bacteria… provides a great volume of extremely useful information and should be used in every laboratory working with plant pathogenic bacteria.”
—Journal of Plant Protection Research

“At its price, this book ought to be a welcome addition to the library of any diagnostic laboratory for plant pathogenic bacteria.”
—New Zealand Journal of Crop and Horticultural Sciences