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Plant Pathology: Techniques and Protocols
Plant Pathology: Techniques and Protocols
In this book expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid.
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Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will be indispensable, both to novices and expert researchers alike.

Features include:

  • Provides researchers with innovative methodologies for plant pathogen diagnostics
  • Written by experts from internationally renowned institutions
  • Cross-cutting approach to diagnostics, including molecular, serological, and traditional methodologies
  • All classes of plant pathogen covered in methodologies
  • Provides methods for crop plant genetic validation

Plant Pathology: Techniques and Protocols


Preface
Contributors

Chapter 1: Bioassay for Diagnosis of Plant Viruses

Isla A. Browning


Chapter 2: Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection

Hei-ti Hsu


Chapter 3: Immunisation Strategies for Antibody Production

Robert Burns

Chapter 4: Preparation of Immunogens and Production of Antibodies

Hei-ti Hsu, Tsung-chi Chen, Chin-an Chang, and Shyi-dong Yeh

Chapter 5: Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience

Dirk U. Bellstedt

Chapter 6: Production of Monoclonal Antibodies to Plant Pathogens

Christopher R. Thornton

Chapter 7: Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria

Blanka Kokoskova and Jaap D. Janse

Chapter 8: Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria

Jaap D. Janse and Blanka Kokoskova

Chapter 9: Detection of Four Major Bacterial Potato Pathogens

Ellen M. Kerr, Greig Cahill, and Karen Fraser

Chapter 10: Erwinia amylovora: Modern Methods for Detection and Differentiation

Antonet M. Svircev, Won-Sik Kim, Susan M. Lehman, and Alan J. Castle

Chapter 11: The Use of Fluorescent In Situ Hybridization in Plant Fungal Identification and Genotyping

Mohamed Hijri

Chapter 12: Use of Molecular Methods for the Detection of Fungal Spores

Elaine Ward

Chapter 13: Identification of Phytophthora fragariae var. rubi by PCR

Alexandra Schlenzig

Chapter 14: Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

Nikki D. Charlton, Stellos M. Tavantzis, and Marc A. Cubeta

Chapter 15: Immunocapture-PCR for Plant Virus Detection

Vincent Mulholland

Chapter 16: Multiplex Polymerase Chain Reaction (PCR) and Real-Time Multiplex PCR for the Simultaneous Detection of Plant Viruses

V. Pallas, J. Sanchez-Navarro, A. Varga, F. Aparicio, and D. James

Chapter 17: Fluorescent-Based Techniques for Viral Detection, Quantification, and Characterization

Mathieu Rolland, Agnès Delaunay, and Emmanuel Jacquot

Chapter 18: Analysis of Population Structures of Viral Isolates Using Single-Strand Conformation Polymorphism Method

Agnès Delaunay, Mathieu Rolland, and Emmanuel Jacquot

Chapter 19: Direct Detection of Plant Viruses in Potato Tubers Using Real-Time PCR

Neil Boonham, Lynn Laurenson, Rebecca Weekes, and Rick Mumford

Chapter 20: An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil

Triona Davey

Chapter 21: Detection of Phytoplasmas of Temperate Fruit Trees

Margit Laimer

Chapter 22: PCR Detection of Potato Cyst Nematode

Alex Reid

Chapter 23: Potato Cultivar Genome Analysis

Alex Reid, Lysbeth Hof, Danny Esselink, and Ben Vosman

Chapter 24: Barley Variety Identification Using SSRs

Cathy Southworth


Index
Publish Date: 2009
Format: 7.5" x 10.3" hardcover
Pages: 326
Images: 42 images
Publication Weight: 3 lbs

Edited by Robert Burns

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